ngs zootsy-comm downstream primer (Azenta)
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ngs zootsy-comm downstream primer
Figure S5 H. (C) ddPCR experiments showing the insertion frequency of AjTn6022 into pTarget with a 200 bp-fragment of comM (left) and E. coli endogenous comM (right) in the absence of TnsF and/or presence of the pTarget(AjTn6022). pSC101 donor was used. (D) ddPCR experiments showing the insertion frequency of ZooTsy into pTarget with a 200 bp-fragment of comM (left) and E. coli endogenous comM (right) in the absence of TnsF and/or presence of the pTarget(ZooTsy). pSC101 donor was used. (E) Electrophoretic mobility shift assay (EMSA) to assess the interaction between a 200-bp or 200-nt fragment of AjcomM and purified AjTn6022-TnsF. (F) EMSA to assess the interaction between a 200-bp or 200-nt fragment of ZoocomM and purified ZooTsy-TnsF_Y584F. (G) Insertion sites of Tn6022 and ZooTsy on E. coli endogenous comM and comM of their respective hosts used in the pTarget. ComM protein sequences (translated comM ) are shown below the nucleotide sequence. The pink rectangle indicates the genomic location of the Walker B of the AAA ATPase encoded by comM ; the red rectangle shows the probable hot spot binding region of both TnsFs. (H) Model of TnsF target selection and insertions for Tn6022 and Tsy. ddPCR experiments were performed with three biological replicates. All data points are shown with an error bar showing standard deviation, and statistical significance was assessed by t test. ∗ p < 0.05; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; n.s., not significant. See also Figures S5–S7 . " width="250" height="auto" />
Ngs Zootsy Comm Downstream Primer, supplied by Azenta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+zootsy-comm+downstream+primer/ngs+zootsy+comm+downstream+primer/pmc10293859-91-5-3
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Figure S5 H. (C) ddPCR experiments showing the insertion frequency of AjTn6022 into pTarget with a 200 bp-fragment of comM (left) and E. coli endogenous comM (right) in the absence of TnsF and/or presence of the pTarget(AjTn6022). pSC101 donor was used. (D) ddPCR experiments showing the insertion frequency of ZooTsy into pTarget with a 200 bp-fragment of comM (left) and E. coli endogenous comM (right) in the absence of TnsF and/or presence of the pTarget(ZooTsy). pSC101 donor was used. (E) Electrophoretic mobility shift assay (EMSA) to assess the interaction between a 200-bp or 200-nt fragment of AjcomM and purified AjTn6022-TnsF. (F) EMSA to assess the interaction between a 200-bp or 200-nt fragment of ZoocomM and purified ZooTsy-TnsF_Y584F. (G) Insertion sites of Tn6022 and ZooTsy on E. coli endogenous comM and comM of their respective hosts used in the pTarget. ComM protein sequences (translated comM ) are shown below the nucleotide sequence. The pink rectangle indicates the genomic location of the Walker B of the AAA ATPase encoded by comM ; the red rectangle shows the probable hot spot binding region of both TnsFs. (H) Model of TnsF target selection and insertions for Tn6022 and Tsy. ddPCR experiments were performed with three biological replicates. All data points are shown with an error bar showing standard deviation, and statistical significance was assessed by t test. ∗ p < 0.05; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; n.s., not significant. See also Ngs Zootsy Comm Downstream Primer, supplied by Azenta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+zootsy-comm+downstream+primer/ngs+zootsy+comm+downstream+primer/pmc10293859-91-5-3
Average 90 stars, based on 1 article reviews
ngs zootsy-comm downstream primer - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Modularity and diversity of target selectors in Tn7 transposons"
Article Title: Modularity and diversity of target selectors in Tn7 transposons
Journal: Molecular Cell
doi: 10.1016/j.molcel.2023.05.013
Figure S5 H. (C) ddPCR experiments showing the insertion frequency of AjTn6022 into pTarget with a 200 bp-fragment of comM (left) and E. coli endogenous comM (right) in the absence of TnsF and/or presence of the pTarget(AjTn6022). pSC101 donor was used. (D) ddPCR experiments showing the insertion frequency of ZooTsy into pTarget with a 200 bp-fragment of comM (left) and E. coli endogenous comM (right) in the absence of TnsF and/or presence of the pTarget(ZooTsy). pSC101 donor was used. (E) Electrophoretic mobility shift assay (EMSA) to assess the interaction between a 200-bp or 200-nt fragment of AjcomM and purified AjTn6022-TnsF. (F) EMSA to assess the interaction between a 200-bp or 200-nt fragment of ZoocomM and purified ZooTsy-TnsF_Y584F. (G) Insertion sites of Tn6022 and ZooTsy on E. coli endogenous comM and comM of their respective hosts used in the pTarget. ComM protein sequences (translated comM ) are shown below the nucleotide sequence. The pink rectangle indicates the genomic location of the Walker B of the AAA ATPase encoded by comM ; the red rectangle shows the probable hot spot binding region of both TnsFs. (H) Model of TnsF target selection and insertions for Tn6022 and Tsy. ddPCR experiments were performed with three biological replicates. All data points are shown with an error bar showing standard deviation, and statistical significance was assessed by t test. ∗ p < 0.05; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; n.s., not significant. See also Figure Legend Snippet: TnsF targets a conserved Walker B motif in comM (A) Schematic of the locus architecture of Zoogloea sp. LCSB751 target selector based on tyrosine (Y) recombinase transposon (ZooTsy). (B) Genetic requirement of YRec, HTH, and TnsF on ZooTsy transposition activity, as assayed by quantification of upstream-end1 junction formation by ddPCR. Deleted genes are indicated by a dashed outline. See also
Techniques Used: Activity Assay, Electrophoretic Mobility Shift Assay, Purification, Sequencing, Binding Assay, Selection, Standard Deviation
Figure Legend Snippet:
Techniques Used: Recombinant, Staining, DNA Purification, Purification, Plasmid Preparation, Mutagenesis, Ligation, Sequencing, Protease Inhibitor, Software
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